Microfluidic Extraction of Rna from Blood
نویسندگان
چکیده
We demonstrate a novel assay for purification of RNA (16S rRNA) from whole human blood infected with Pseudomonas Putida (P. Putida). This assay is unique in that the extraction chemistry provides a method which both lyses bacteria cells and protects RNA from degradation when isolating from matrices rich with ribonuclease (RNase) (such as blood). The method can also be integrated with on-chip RNA purification using isotachophoresis (ITP). We demonstrate that the extracted 16S rRNA is compatible with reverse transcription polymerase chain reaction (RT-qPCR) and detect P. putida infected whole blood at clinically relevant bacterial cell concentration of 30-30,000 bacteria per microliter of blood.
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